반응형
What type of signals do we see with Flow ?
Scatter
– Forward Scatter (FSC)
parallel or Perpendicular FSㅊ
– Side scatter (SSC)
Fluorescence
– FITC , PE, APC, GFP, DAPI (plus lots lots more)
Scatter parameters & how they are measured
Understanding Scatter Signals
FSC has some similarities to size
SSC has some similarities to granularity and complexity
Fluorescent Signals: A cytometer can detected light from any system you can design that utilises fluorescence
Fluorescence may be used in the detection of :
–Protein, RNA and DNA
–DNA synthesis
–Dye efflux
–Organelle Activity
–Change in pH
–Protein interactions
–Cell movement and division
–etc
반응형
'실험노트 > Flow cytometry' 카테고리의 다른 글
| Flow Cytometry 3 core systems (0) | 2019.06.26 |
|---|---|
| Introduction to FlowCytometry (0) | 2019.06.25 |
